Journal of Pure and Applied MicrobiologyVol. 8 No. 2

Correlation Between Phage Typing and Toxins Content as an Outbreak Tool in Staphylococcus aureus

Manal M. AlKhulaifi, Nagwa M. Aref*, Ali A. AlSalamah and Mohammed. S. Al Shammary

Department of Botany and Microbiology, College of Science, King Saud University, P.O.Box 22452.Riyadh 11495, Kingdom of Saudi Arabia.

Received on 26 August 2013 and accepted on 02 November 2013

 

ABSTRACT

Staphylococcus aureus is one of the major causes of community and hospital acquired infections. As well as bacteriophage considered as a major risk factor acquires S. aureus new virulence genetic elements for it. A total number of 119 S. aureus isolates obtained from Riyadh Military Hospital. And were studied for phage typing and the incidence of toxin genes by PCR. Methicillin Resistant S. aureus isolates (MRSA) indicated high special prevalence of phage group II with a highly increase for phage type ?3A compared to MSSA. Phage group II on Methicillin Sensitive S. aureus isolates (MSSA) considered an epidemiologic marker with frequent strong reaction compared to group III and phage group I. Phage type ?75 may play an important role in a combination with ?80 or/ ?81 by having PVL toxin to be CMRSA lineages. 68% of S. aureus isolates had toxins. The most prevalent toxins were SEO, in 50.8% in MSSA & 25% in MRSA isolates. SEI was detected in 40.3% in MSSA & 29.1% in MRSA isolates. Also, SEA was 28% in MSSA & 33.3% in MRSA isolates. Phenotypic and genotypic variations between MSSA isolates seemed to be horizontally, while in MRSA isolates were vertically. It was obvious that five toxins together were located in more than one isolates in MSSA.

Keywords : Staphylococcus aureus, Phage types, Toxins, epidemiologic marker, MSSA, MRSA.