Journal of Pure and Applied MicrobiologyVol. 8 No. 2

Agrobacterium-Mediated Genetic Transformation and Regeneration of Low-Toxicity Variety of Castor (Ricinus communis L.)

Wei Li1, Guixian Yang1, Yurong Wang1, Zhenjing Li1, Haiyan Cui1 and Changlu Wang1,2*

1Key Laboratory of Food Nutrition and Safety (Tianjin University of Science and Technology), Ministry of Education, Tianjin - 300 457, China. 2School of Food Engineering and Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Avenue, TEDA, Tianjin - 300 457, China.

Received on 09 January 2014 and accepted on 18 March 2014

 

ABSTRACT

Low-toxicity transgenic castor (Ricinus communis) plants were produced through Agrobacterium-mediated genetic transformation. Ricin, which is highly toxic, affects the application of castor. In this research, a 351-bp fragment located at ricin A chain gene (RTA) was selected to establish an RNA interference construct that was introduced into epicotyl of castor, thus transformants were obtained. Through semi-quantitative analysis on RTA, it was confirmed that the RTA expression quantity of the transformants was apparently decreased, demonstrating the interference efficiency of the transformation vectors and their function of reducing the expression of ricin at the RNA level. An improved protocol for the transformation and regeneration of less toxic caster has been developed for the first time.

Keywords : Castor, ricin, Agrobacterium tumefaciens, RNAi