Journal of Pure and Applied MicrobiologyVol. 8 No. 2

Co-microencapsulation Technology to Improve Cell Viability

Xiaolin Tang1, Yuesheng Tong1, Shibin Wang1,2*, Aizheng Chen1,2, Wenguo Wu1,2 and Yuangang Liu1,2*

1College of Chemical Engineering, Huaqiao University, Xiamen - 361 021, China. 2Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen - 361 021, China.

Received on 09 January 2014 and accepted on 18 March 2014



Microencapsulation technology is a useful tool for cell cultivation and metabolite production of bacteria. In this paper, Alg-Sr2+ microcapsules were used to co-culture two kinds of cells to enhance cell viability. Alginate and strontium chloride are introduced to prepare microcapsules for their resistance to immune rejection and perfect biocompatibility. Four groups including HepG2, co-cultured 3T3/HepG2, microencapsulated HepG2 and co-microencapsulated 3T3/HepG2 were prepared to determine the cell viability, albumin secretion and urea synthesis. MTT assay showed the relative growth rate (RGR) of co-cultured 3T3/HepG2 was the highest, co-microencapsulated group was the second and microencapsulated HepG2 was the lowest. Additionally, significant difference was shown on albumin secretion among the four groups. Co-microencapsulated 3T3/HepG2 had the notable albumin secretion. On the contrary, HepG2 group increased slowly and even decreased after five days. Besides, co-cultured or co-microencapsulated cells synthesize more urea than other two groups. The results indicate that 3T3 cells and Alg-Sr2+ microencapsulated environment can promote the viability of hepatocytes, which also provides an alternative way for the future bacterium cultivation to increase the bacterium yield or metabolite production.

Keywords : Alg-Sr2+ microcapsules; co-culture; HepG2; 3T3