Journal of Pure and Applied MicrobiologyVol. 8 No. 2

Biosynthesis of Silver Nanoparticles from Penicillium spinulosum (MTCC-4952) and Evaluation of their Antibacterial Activity in Combination with Amoxicillin and Ofloxacin

Anima Nanda and Shahnaz Majeed

Department of Biomedical Engineering, Sathyabama University, Rajiv Gandhi Salai, Chennai - 600119, India.

Received on 18 February 2014 and accepted on 21 March 2014

 

ABSTRACT

In the recent years Nanotechnology has attracted the great attention to the researchers and pharmaceutical companies due to its immense application in the field of science and technology for the purpose of developing new nanomaterial?s . In our present study, naosilver was bio-synthesized by extracellular method from Penicillium spinulosum (MTCC-4952). The developing of dark brown color in the l flask by addition of AgNo3 indicates the formation of Ag-NPs. The supernatant of the fungus culture alters the solution from colorless to dark brownish color upon the completion of the 20 minute reaction taken place in the solution. These nanoparticles were confirmed by Uv-Vis spectrophotometer which shows absorption peak at 416nm, Fourier transform infrared (FTIR) analysis, Field emission scanning electron microscopy (FESEM) and Atomic force microscopy (AFM). Size of the Nanoparticles was around 18nm by FESEM. Silver nanoparticles showed good antibacterial activity against the selected bacterial pathogens butin combined activity with Amoxicillin and Ofloxacin these biosynthesized Silver Nanoparticles from Penicillium spinulosum (MTCC-4952) enhances the antibacterial property of the antibiotics studied.

Keywords : Silver nanoparticles, Penicillium spinulosum (MTCC4952), FESEM, FTIR, AFM,UV-VIS Spectrophotometer.