Journal of Pure and Applied MicrobiologyVol. 8 No. 2

Resistance Analysis of Lilium Oriental on Bulb Rot

Songping Hu1*, Zhigang Wei1*, Sixiang Zheng2, Yuanyauan Nie3, Lin Zhang3**, Tingting Pei1 and Xia Yu1

1College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang City of Jiangxi Province - 330045, China. 2Agricultural Sciences Institute of Zhuzhou, Zhuzhou City of Hunan Province - 412007, China. 3College of Science, Jiangxi Agricultural University, Nanchang City of Jiangxi Province - 330045,China.

Received on 09 January 2014 and accepted on 18 March 2014

 

ABSTRACT

In order to understand the mechanisms of bulb rot pathogenesis and disease resistance of Lilium Oriental as soon as possible, the study was conducted using Lilium Oriental materials from the Agricultural Sciences Institute of Zhuzhou in Hunan. The bulb rot pathogen was isolated by the mercuric chloride disinfection method. Then, the effect of seven inoculation methods and completed disease resistance identification of 30 self-cultivation Lilium Oriental materials were compared. At the same time, the total content and antifungal activity of saponin in materials were also investigated. The results suggested that Fusarium oxysporum was the pathogen causing Lilium Oriental bulb rot. According to the clustering analysis, 30 Lilium materials tested were divided into three groups of 3 resistant, 16 medium resistant and 11 susceptible materials. It also showed that resistant materials had significantly higher saponin content than susceptible materials did. Additionally, the saponin content was significantly correlated to resistance levels (r=0.819). With the mixed liquid plate culture method, it was discovered that the saponin extract solution, whose total saponin content was 1.194mg/mL, had a certain inhibition rate of 29.39% on pathogen?s mycelial growth.

Keywords : Lilium Oriental; bulb rot; Fusarium oxysporum; resistance identification; saponin content.