Journal of Pure and Applied MicrobiologyVol. 8 No. 2

Propagation of Aspergillus niger in Stirred Fermentor for the Production of Glucose Oxidase

Irshad Ahmad1*, Zia Ul Islam2, Zhisheng Yu2 and Muhammad Mohsin Javed3

1Department of Microbiology and Biotechnology, Sarhad University of Science and Information Technology, Peshawar, Khyber Pakhtunkhwa, Pakistan. 2College of Resources and Environment, University of the Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China. 3Institute of Industrial Biotechnology, Government College University, Lahore, Punjab, Pakistan.

Received on 03 July 2013 and accepted on 26 August 2013

 

ABSTRACT

The present research work was planned for enhanced glucose oxidase production in Aspergillus niger by optimizing the growth conditions in a stirred fermentor. The results revealed that medium M2 containing (g/L) glucose 80.0, peptone 3.0, (NH4)2HPO4 0.388, KH2PO4 0.188 and MgSO4.7H2O 0.156 was the best among four tested media. Glucose (8%) was the best carbon source for the maximum yield of glucose oxidase (118 U/g cell mass) followed by sucrose (103 U/g cell mass) and fructose (99 U/g cell mass). (NH4)2HPO4 followed by peptone were the best nitrogen sources for the growth of A. niger and production of glucose oxidase. The maximum activity (118 U/g) of glucose oxidase was produced after 72 hours of fermentation at pH 5.0, temperature 30?C, agitation 200 rpm and aeration of 1 vvm.

Keywords : Aspergillus Niger, Glucose oxidase production, Stirred fermentor.