Journal of Pure and Applied MicrobiologyVol. 8 No. 2

Taguchi's - A Recent Statistical Approach to Optimize the Microbial Pectinases Screened from Fruit Waste Yard

R. Gopinath and Suneetha V.

School of Bio Sciences and Technology, VIT University,Vellore-632 014, Tamil Nadu, India.

Received on 06 June 2013 and accepted on 01 September 2013

 

ABSTRACT

Pectinase posses potent industrial application and hence pectinolytic bacteria Bacillus cereus (KC571175) that posses ability to produce pectinase enzyme are of prime importance. The main theme of work concentrates around isolation of potent pectinolytic bacteria from fruit processed industrial waste to aid in wide commercial application and to recycle the waste to produce a clean and sustainable environment. Series of processes in a calculated way to carry out careful fermentation has led to production of enzymes that show high activity. The Taguchi method is a simple statistical tool to design the experiments involving a system. The orthogonal array is an unbiased. The experimental runs were carried based on the factors and levels. To predict the significant contribution of the each factors signal-to-noise ratio was used. It shows a signification different in various levels of the factors. At Temperature 39?C, pH 5, RPM 75, Pectin 7% and Incubation (in hours) 120 the maximum activity of polygalacturonase and pectin lyase were observed.

Keywords : Pectin lyase, Polygalacturonase, Taguchi, SN ratio, Bacillus cereus.