Journal of Pure and Applied MicrobiologyVol. 6 No. Special Edition Oct. 2012

Study the Prevalence of Hepatitis B and Evaluation of Two Rapid Immunochromatographic Tests for the Detection of Human Hepatitis B Surface Antigen in a Tertiary Care Hospital in New Delhi, India

S. Nandwani, M. Choudhry, S. Misra and S. Gupta

Department of Microbiology, ESIC- PGIMSR & Hospital, Basaidarapur, New Delhi - 110 015, India.

Received on 04 March 2012 and accepted on 10 June 2012

 

ABSTRACT

Hepatitis B viral (HBV) infection is a leading cause of liver disease worldwide. ?This study was done to see the prevalence of hepatitis B in patients reporting at our hospital and evaluation of two rapid Immunochromatographic testing (ICT) kits for HBs Ag detection. A total no. of 5024 serum samples were collected wef Jan 2011 to July 2011 from patients and were tested for HBs Ag by ELISA. The two ICT tests- InstachkTM by InTec Products INC and HEPATMCARD by Reckon Diagnostcs Pvt. Lt. were performed on Elisa tested samples for HBV- Reactive (n = 137) and HBV- Non reactive (n = 150) samples. Prevalence of Hepatitis B in our study was found to be 2.73 %, 137 being reactive out of 5024 samples by ELISA. The I.C.T. results showed a sensitivity of 94.16 % and 51.09%, specificity of 100% and 96.67 %, positive predictive values of 100% and 93.33 % while the negative predictive values of 94.93 % and 68.39 % for InstachkTM and HEPATMCARD kits respectively. These results suggest that the rapid ICT kits for HBsAg have only limited efficacy and should be backed by superior methods like ELISA and PCR where possible.

Keywords : Prevalence, HBV, hepatitis, ELISA, Immunochromatographic tests, HBs Ag.