Journal of Pure and Applied MicrobiologyVol. 6 No. Special Edition Oct. 2012

Efficacy of Neem (Azadirachta indica A. Juss) Seed Kernel Extract against Dengue Vector, Aedes aegypti (Diptera: Culicidae)

Shah M. Haris* and Absar M. Khan

Department of Zoology, Aligarh Muslim University, Aligarh - 02, India.

Received on 04 March 2012 and accepted on 10 June 2012



Laboratory bioassays were conducted to evaluate the larvicidal, ovicidal and repellent activity of neem (Azadirachta indica A. Juss) seed kernel extract (NSKE) on Aedes aegypti. The neem seed kernel powder was sequentially extracted with ethyl acetate, acetone, ethanol and distilled water. The extract exhibited dose dependent activity and produced significant mortality. The 24 h LC50 concentration of the extract was observed at, 11.45, 19.95 and 24.67 mg/l respectively. Mean per cent hatchability of the ovicidal activity was observed 120.00 h after treatment. The per cent hatchability was inversely proportional to the concentration of extract and directly proportional to the eggs. The crude extract of neem seed kernel shows significant repellency against A. aegypti. These results clearly reveal that NSKE served as a potential larvicidal, ovicidal and repellent agent against dengue vector mosquito, A. aegypti.

Keywords : Azadirachta indica, Aedes aegypti, Larvicidal activity, Ovicidal activity, Repellent activity.