Journal of Pure and Applied MicrobiologyVol. 6 No. Special Edition Oct. 2012

Serodiagnostic and Clinical Presentation of Dengue Virus Infection in A Tertiary Care Hospital

Suchitra Jain*#, Trupti Bajpai#, G.S.Bhatambare and A.B. Deshmukh

Department of Microbiology, Shri Aurbindo Medical College, Ujjan-Indore Highway, Indore, India.

Received on 04 March 2012 and accepted on 10 June 2012

 

ABSTRACT

The scale and intensity of present outbreak confirms that a new paradigm of Dengue disease, a complex in the form of Dengue Hemorrhagic Fever and Dengue Shock Syndrome has now secured a firm foothold in India. The new lethal manifestation of an old benign disease broke out in the Central India. The objective of the study was to evaluate the utility of serodiagnosis of Dengue virus infection and correlate the serological results with clinical presentations in patients diagnosed to be suffering from dengue at a tertiary care hospital in Indore, Madhya Pradesh, Central India. Laboratory diagnosis of Dengue Virus infection mainly depends on detection of Antigen or virus specific Antibodies. The patients admitted with Dengue-like symptoms were screened for their seropositivity by Rapid Immunochromatographic and ELISA methods and the results were correlated clinically. Out of the 585 patients tested, 317 were seropositive. About 46.37% patients tested positive for Dengue NS1 Antigen and 57.41% patients tested positive for IgM antibody. The patients presented with a range of signs and symptoms indicative of different phases of disease. Hundred percent patients categorized under DHF and DSS were found to be in the acute phase of infection. Most of the reported cases occurred in young adults. Hence it was concluded that rapid, accurate and early serodiagnosis of dengue virus infection from acute phase viremic blood samples from patients in febrile stage contributes greatly to patient management in hospitals and control measures in public health.

Keywords : Dengue Hemorrhagic Fever, Dengue Shock Syndrome, Dengue Antigen, Dengue IgM.