Journal of Pure and Applied MicrobiologyVol. 3 No. 2

Effect of UV and EMS Mutation on Aspergillus niger in the Production of a-amylase

C. Mahalakshmi¹, S. Miller Samson²*, S. Alagendran², R. Anusha² and E. Neelamathi²

¹Department of Biotechnology, Bharath College of Science and Management, Thanjavur. ²PG Department of Biotechnology, Nehru memorial College (Autonomous), Puthanampatti - 621 007, India.

Received on 13 July 2009 and accepted on 21 April 2009

 

ABSTRACT

Production of extracellular a- amylase enzyme by a filamentous fungus, Aspergillus niger was studied in rice submerged fermentation. The potential strain was successfully mutated by ultraviolet (UV) and ethyl methane sulphonate (EMS). High level of a- amylase activity was obtained by the mutant strains UV-3 and EMS-2. These strains exhibited 1.44 and 2.19 times increased production of a- amylase respectively than the wild strain, which reveals the potential use of mutant strains in the industries.

Keywords : Aspergillus niger, a- amylase, EMS, UV, Mutation, Ascomycetous fungi.