Journal of Pure and Applied MicrobiologyVol. 6 No. Special Edition Oct. 2012

Prevalence of Torque Teno Virus (TTV) Infection in Liver Disease Patients

Shafquat Jahan?*, Meher Rizvi?, Md. Azam?, M.R.Ajmal?,Indu Shukla? and Abida Malik?

?Department of Microbiology,Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, India. ?Department of Medicine, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, India.

Received on 04 March 2012 and accepted on 10 June 2012



To investigate the prevalence of Torque Teno virus (TTV) in patients with acute and chronic liver disease, present at a tertiary care hospital in north India. The present study was conducted on 135 patients over a period of one and half year. Detailed clinical history was elicited such as fever, anorexia, nausea, vomiting, abdominal discomfort, high coloured urine, weight loss, any past H/O jaundice, alcohol intake , blood transfusion and H/O pregnancy. MELD (Model for End Stage Liver Disease) scoring was done to evaluate the extent of liver damage.DNA extraction was done from patient?s sera by chloroform-ethanol technique. Nested PCR was performed using predesigned primers (by fermentas) . TTV DNA bands were seen on agarose gel electrophoresis. 20 cases (15%) were positive for the TTV DNA. Of which 12 had acute viral hepatitis(AVH),while 6 had chronic viral hepatitis (CVH) and 2 of them had Cirrhosis. Mean levels were AST 49.5 IU/L, ALT 65.75 IU/L , ALP 40.38 KAU, Total Bilirubin 6.95 mg%, and INR 2.32 respectively. The mean of MELD Score was 18.30 ranging from 9 to 36. MELD Score above 20 were found in 6 patients of acute liver disease, and 2 patients of chronic liver disease. TTV prevalence in liver disease is significant though the real clinical impact is still unclear.

Keywords : TTV Virus, Liver disease, Prevalence, Clinical.