Journal of Pure and Applied MicrobiologyVol. 3 No. 2

Biological Degradation of Herbicide (Atrazine) using Pseudomonas aeruginosa and Trichoderma viridae

M. Muthuselvam and S. Arunkumar*

Muthaiyah Research Foundation, Thanjavur, Tamil Nadu, India.

Received on 21 March 2009 and accepted on 03 May 2009

 

ABSTRACT

Herbicides are widely used in agriculture. Asia now accounts for a vast majority of the global rice herbicide market. In this study bacteria and fungal species was isolated from the soil samples. Among these isolated colonies Pseudomonas aeruginosa and Trichoderma viridae were identified based on the cultural, morphological and biochemical characteristics. The herbicide (atrazine) degradation ability of Pseudomonas aeruginosa and Trichoderma viridae were analyzed. Among the study highest growth were noted in atrazine containing medium for both test organism. Maximum growth indicates the highest degradation of herbicides. The effect of herbicide was noted based on the morphometric and biochemical characteristics of the experimental plant (Vigna mungo). The decreased Seed germination, shoot and root length noted in Herbicide containing pot when compared with other treatment. Biochemical compound such as chlorophyll, and total protein were analyzed. Among the study all the biochemical parameters such as chlorophyll, protein content decreased amount noted in Herbicide containing pot, when compared with control treatment (without Herbicide).

Keywords : Herbicide, Soil, Pseudomonas aeruginosa, Trichoderma viridae and Biological degradation.