Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Capsule Treatment by Soluble Expressed Recombinant Heparinase II Enhance the Foreign Gene Transformation to Escherichia coli K5

Haichan Huang1, Shencong Lv1, Lei Zhao1, Ziqin Yan1, Weihong Zhong1* and Fuming Zhang2

1College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, PR China. 2Department of Biochemical Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.

Received on 23 September 2013 and accepted on 06 November 2013

 

ABSTRACT

In the present study, a recombinant E. coli BL21 strain containing heparinase II gene was utilized to express and purify soluble heparinase II. When incubated at a low culture temperature of 22?C and in an auto-induction medium, the recombinant E. coli BL21 could successfully enhance the express of heparinase II in soluble and active forms. The purified recombinant heperinase II could eliminate heparosan in vitro completely and de-polymerized in vivo the capasule heparosan of E coli K5. Heparinase II treatment on E. coli K5 capsular polysaccharide enhanced the transformation efficiency of foreign genes into K5. It will improve the metabolic engineering operation in this strain. To our knowledge, it was first report to utilize heparinase II to eliminate K5 capsule.

Keywords : Heparinase II, heparosan, Depolymerization, Capsule, E coli K5, Gene transformation.