Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Classification of Potassium-releasing Bacteria Isolated from Four Agricultural Soil Samples

Xiaolu Liu1, Yongzhi Liu1, Liuqing Yang1, Tao Guo2, Le Lv1, Chunhua Yin1, Qianqian Xu1, Feng Zhou1 and Hai Yan1*

1School of Chemistry and Biological Engineering, University of Science and Technology Beijing, 30 XueYuan Road, Hai Dian District, Beijing - 100 083, China. 2Shandong Rice Research Institute, Shandong Academy of Agriculture Sceneces, Jinan - 250 100, China.

Received on 23 September 2013 and accepted on 06 November 2013

 

ABSTRACT

Bacteria capable of potassium-releasing are spread among phylogenetically diverse groups. The purpose of this work was to study the microbial diversity and potassium-releasing capacity of potassium-releasing strains isolated from four rhizosphere soil samples of tobacco from Luzhou, Sichuan Province, China. The density of potassium-releasing bacteria in the S soil sample was significantly higher than in others. Potassium-releasing bacteria isolated from four soils were grouped into 18 strains according to ARDRA. One colony was picked to culture and sequencing from each of the 18 strains to construct the phylogenetic tree, and the tree obtained from the sequences revealed four major branches exhibiting highest identity to the following genera: (i) Bacillus, (ii) Enterobacter, (iii) Pseudomonas and (iv) Providence. Majority of the strains with high potassium-releasing efficiency belong to Bacillus, and JK01 (Bacillus mojavensis) possesses the highest capability of potassium-releasing (95.01% higher than the control), it has greater potential to serve as plant growth promoting rhizobacteria.

Keywords : Soil, Potassium, Isolation, Classification, Capability.