Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Antifungal Activity of Citrullus colocynthis against Fusarium oxysporum, Alternaria alternata, Macrophomina phaseolina and Colletotrichum musae

Najat A. Bokhari, Kahkashan Perveen*, Iffat Siddiqui, Iram Siddiqui, Mona S. Wahibi and Dina A.W. Soliman

Department of Botany and Microbiology, King Saud University, P.O. Box 22452, Riyadh-11495, Kingdom of Saudi Arabia.

Received on 15 August 2013 and accepted on 05 October 2013

 

ABSTRACT

Four important plant pathogenic fungi were subjected to evaluate the antifungal potentiality of Citrullus colocynthis. It was recorded that the growth of Fusarium oxysporum, Alternaria alternata, Macrophomina phaseolina and Colletotrichum musae were significantly (Pd? 0.01) reduced by extract of C. colocynthis obtained by various solvents (water, acetone, ethanol, methanol and chloroform). Ethanolic extract gave the most promising results against all tested fungi. M. phaseolina was observed to be highly sensitive to the ethanol extract and zone of inhibition was 26.5 mm. Ethanolic extract rendered highest the percent growth reduction of M. phaseolina (41.67%). The growth F. oxysporum, C. musae and A. alternata were reduced by 37.2%, 35.67% and 28.935% respectively by the ethanolic extract of C. colocynthis. GC-MS analysis of crude extract showed that the major components of the crude extract were l-(+)-Ascorbic acid 2,6-dihexadecanoate (94.2%), Eicosanoic acid (92.3%) and 2-Heptadecenal (91.8%).

Keywords : Antifungal activity, Citrullus colocynthis, Plant pathogenic fungi, GC-MS.