Journal of Pure and Applied MicrobiologyVol. 7 No. 4

PCR–based subtyping of Blastocystis isolates from symptomatic and asymptomatic patients in North-West of Iran

Abbas Shahbazi1, Esmaeil Fallah2, Peyman Heydarian1, Ardavan Ghazanchaei2, Majid Khanmohammadi3, Nasrin Mirsamdi4

1Tabriz Research Center of Infectious and Tropical Diseases, Tabriz University of Medical Sciences, Tabriz, Iran
2 Department of Medical Parasitology and Mycology, Tabriz University of Medical Sciences, Tabriz, Iran
3Department of Laboratory Sciences, Marand Branch, Islamic Azad University, Marand, Iran
4Tabriz Central Laboratory, Tabriz University of Medical Sciences, Tabriz, Iran

Received on 11 March 2013 and accepted on 29 May 2013

 

ABSTRACT

The pathogenic potential of Blastocystis hominis is still controversial. To find the pathogenicity of this organism determining the genotypic differences among symptomatic and asymptomatic isolates can be useful. To determine genetic diversity of Blastocystis hominis in North West of Iran, 57 isolates from 34 asymptomatic healthy individuals and 23 symptomatic patients were genotyped by polymerase chain reaction using seven pairs of known subtype specific sequence tagged site (STS) primers. Out of 57 isolates, ST3 as predominant (50.87%) followed by ST1 (40.35%) and ST2 (8.77%) were recognized. By comparing genotype of Blastocystis isolates among asymptomatic and symptomatic group, we found that in asymptomatic group subtype 3 was most dominant (29/34) and all patients in symptomatic group classify as subtype 1 (23/23).

Keywords : Blastocystis hominis, PCR, Genotype, Iran.