Journal of Pure and Applied MicrobiologyVol. 7 No. 4

In vitro Biological Activity of Liquidambar orientalis Mill against Pathogen and Marine Biofilm Microorganisms

Sibel Avunduk1* and Asli Kacar2

1Medical Laboratory Techniques Programme, Vocational School of Health Care, Mugla University, Marmaris, Mugla, 48187 Turkey. 2Institute of Marine Sciences and Technology, Dokuz Eylul University, Baku Bul. No: 100 Inciralti, Izmir, Turkey.

Received on 15 March 2013 and accepted on 19 May 2013

 

ABSTRACT

The balsam ?liquid storax? of Liquidambar orientalis Mill. that (Sigla) is an endemic tree species for Eastern Mediterranean, was obtained from G?nn?cek (Marmaris). It was extracted with n-hexane, CH2Cl2 and MeOH respectively. The n-hexane, CH2Cl2, MeOH extracts were tested at concentrations of 10.0%, 1.0%, 0.4%, 0.2% and 0.1% against two different groups of bacteria: Pathogens and marine biofilm bacteria. Klebsiella pneumonia, Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, Pseudomonas aeroginosa, Bacillus cereus, Enterococcus faecalis and Candida albicans were used as pathogen microorganisms whereas, Pseudoalteromonas marina P. haloplanktis, Alteromonas alvinella, Alteromonas genoviensis Vibrio splendidus, Exiguobacterium homiense, Vibrio lentus were used as marine biofilm bacteria. The antimicrobial test results showed that the MeOH extract of storax has more potent antimicrobial activity than CH2Cl2 and n-Hexane extracts. Inhibitory activities of L. orientalis resin extracts were high against biofilm bacteria, while they were found to be low against pathogenic microorganisms tested.

Keywords : Liquidambar orientalis Mill., Medicinal plant, Pathogen and biofilm bacteria.