Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Isolation and Characterization of Antimicrobial Compound from Bacillus sp. Associated with Gastropods

S. Mary Josephine Punitha1*, N. Viju1, S. Sharmin Vini1, C.V. Sunjaiy Shankar2, M. Michael Babu1 and T. Citarasu1

1Center Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, Kanyakumari, India. 2Department of Biotechnology, Udhaya College of Arts and Science, Velamodi, Kanyakumari, India.

Received on 28 September 2013 and accepted on 15 November 2013

 

ABSTRACT

Screening of marine bacteria isolated from the marine environment has made a clear path for the identification of new antimicrobial metabolites. This study was carried out assess the antibacterial activity of gastropod associated bacteria. Gastropods were collected from west cost of India and a total of seven strains were isolated from the surface of it. The antimicrobial activity of the (EPS) Extracellular Polymeric Substances of the isolated strains were tested against four target bacteria Galionella sp., Alteromonas sp., Pseudomonas sp., Klebsiella sp. All the strains showed strong activity. The best active strain was selected and the active compound was characterized by HPLC analysis. The active strain was tentatively identified as Bacillus sp. based on morphological and biochemical characters. These bioactive compounds producing strains could be used as the source for the drug development in pharmaceuticals and industries.

Keywords : Antimicrobial activity, Bioactive compounds; Surface-associated microorganism.