Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Study on Ultrastructure and Antibacterial Activity of Lactarius sanguifluus (Paulet) Fr

Anand Sagar and Kavita Thakur

Department of Biosciences, Himachal Pradesh University, Shimla - 5 India.

Received on 26 November 2012 and accepted on 07 January 2013

 

ABSTRACT

The aim of the present investigation was to study ultrastructure (mycelium and spores) of Lactarius sanguifluus (Paulet) Fr. under Scanning Electron Microscope (SEM). Antibacterial activity of methanolic extract of L. sanguifluus was investigated in-vitro against two pathogenic bacteria Staphylococcus aureus and Escherichia coli following agar well diffusion method, using four different concentrations (25, 50, 75, 100%). The methanolic extract of the mushroom showed significant reduction in the growth of the tested bacteria. The extract showed maximum inhibitory growth against E. coli as compared to S. aureus. There is need of further studies to isolate and characterize the antibacterial moieties in this mushroom for practical disease control measure.

Keywords : Lactarius sanguifluus, SEM, antibacterial, In-vitro, S. aureus, E. coli.