Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Characterization of the b-Tubulin Genes from Stemphylium Species

Daye Huang1, Yanxia Shi2, Xuewen Xie2, Weiping Wang1 and Baoju Li*2

1College of Plant Protection, Shenyang Agricultural University, Shenyang , Liaoning Provinces, China. 2 Insititute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.

Received on 25 November 2012 and accepted on 29 December 2012

 

ABSTRACT

The b-tubulin genes from three Stemphylium species which are insensitive to high concentration of carbendazim were amplified with two pairs of primers and sequenced. The lengths of three genes are 1431bp for Stemphylium solani, 1431bp for Stemphylium lycopersici and 1424bp for Stemphylium spinaciae respectively. All genes have three introns and encode proteins with 398 amino acids. The protein sequences of b-tubulins of three Stemphylium species are highly homologous to those of other plant pathogenic fungi that are sensitive to carbendazim. The main difference is that the 167th amino acid of b-tubulins in Stemphylium is the residue Tyr instead of Phe, which is commonly found at the same position of b-tubulins in other fungi. These results suggest that carbendazim insensitive of the three Stemphylium species probably attributes to Tyr167.

Keywords : b-Tubulin, Amino acid, Insensitive, Stemphylium.