Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Bioaugmentation of Oil-degradation Bacteria Isolated from the Petroleum-contaminated Soil

Lina Qu1,2, Mingming Tu1, Hongbai Jia1 and Qiuyu Wang1*

1Department of Lifescience, Northeast Forestry University, 150040,Harbin, Heilongjiang, China. 2Department of Lifescience, Daqing Normal University, 163712, Daqing, Heilongjiang, China.

Received on 23 September 2013 and accepted on 06 November 2013

 

ABSTRACT

Five bacterial strains isolated from the long-term petroleum-contaminated soil in the Daqing oilfield were used to bioremediated the petrol-oil pollution in soil. They were identified as Pseudomonas sp., Pseudomonas aeruginosa, Acinetobacter junii(1), Acinetobacter1 and Microbacterium oxydans. The results of oil-degrading efficiency showed that under both liquid and solid (i.e. soil) culture conditions, the five bacterial strains showed high petroleum hydrocarbon degradation ability. By regulating the concentration of initial salt, nitrogen and phosphorus in the medium, we explored the optimal conditions for growth and crude oil-degradation of the bacterial strains. The strain X3 showed higher petroleum degradation rate in both of 1% and 3% salt concentration, more than 60%. And the highest oil degradation rate required NH4NO3 and K2HPO4?3H2O /KH2PO4 in mineral medium. This study provides new bacterial resources and information for the autochthonous bioaugmentation of oil contaminated soil.

Keywords : Autochthonous bioaugmentation, Oil-contaminated soil, Petroleum hydrocarbon, Bacteria, Degradation rate.