Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Antimicrobial Activity of Bacillus Strains Isolated from Spring Water and A Novel Bacteriocin: RS108

Elif Sevim1,2, Sengul Alpay Karaoglu1*, Ali Sevim1,3 and Sabriye Canakci4

1Department of Biolgy, Faculty of Arts & Sciences, Rize University, 53100 Rize, Turkey. 2Department of Food Engineering, Faculty of Engineering and Architecture, Ahi Evran University, 40100 Kirsehir, Turkey. 3Department of Environmental Engineering, Faculty of Engineering and Architecture, Ahi Evran University, 40100 Kirsehir, Turkey. 4Department of Biology, Faculty of Sciences, Karadeniz Technical University, 61100 Trabzon, Turkey.

Received on 02 March 2013 and accepted on 21 April 2013

 

ABSTRACT

The aim of this research was to investigate the bacteriocins produced by Bacillus strains isolated from the spring water of Rize, in Turkey. Bacillus cereus RS108 was identified by both conventional and molecular methods, bacteriocin RS108 which was produced by RS108, was partially characterized. A broad range of indicator strains, including several species of bacteria and yeast like fungi, was inhibited by a crude bacteriocin obtained from culture supernatant fluid. The best antimicrobial activity of RS108 was detected on S. pyogenes, L. monocitogenes, and another B. cereus strains, and at the late exponential growth phase. RS108 was stable at 90?C, but the activity was lost when the temperature reached 100 ?C. It was inactivated by proteinase K. It was resistant 10% ration of some solvent (chloroform, ethanol etc.), but sensitive high concentration. Bacteriocin activity was observed in the pH range of 3.0-9.0. SDS-PAGE analysis of the partially purified bacteriocin shows that the molecular weight of bacteriocin RS108 is approximately 4 kDa.

Keywords : Bacillus cereus, Bacteriocin, Spring water, Partial Purification.