Journal of Pure and Applied MicrobiologyVol. 2 No. 2

Antimicrobial Screening and Phytochemical Analysis of Blumea oblique

Aruna Bhagat¹, Aqueel Khan¹ and Gopal Gond²

¹PGTD Biochemistry, RTM University Nagpur, L.I.T. Premises Nagpur - 440 033, India. ³Guru Nanak College of Science, Ballarpur - 442 701 (India)

Received on 06 April 2008 and accepted on 15 August 2008

 

ABSTRACT

Whole plant of Blumea oblique was studied for antimicrobial properties and phytochemical analysis. Aqueous extraction of whole plant was done for preliminary antimicrobial screening. Soxhlet extracts of plant were made in petroleum-ether, chloroform, acetone and methanol and tested against microorganisms. Study found that the aqueous extracts and soxhlet methanolic extracts showed antimicrobial activity. Comparatively higher activity was observed in methanolic extracts. Agar well diffusion technique was used to find out antimicrobial activity of whole plant extracts. Minimum inhibitory concentration (MIC) results confirm bacteriosidal and bacteriostatic activity at low and high dilution rate. Phytochemical analysis and various confirmatory tests have revealed the presence of alkaloids, cardiac glycosides, steroids and flavonoids. The present study found that bacteriosidal and bacteriostatic activity in Blumea oblique thus, the presnt study suggests that this plant is used as an antimicrobial agent.

Keywords : Blumea oblique, antimicrobial property, MIC, Agar well diffusion, phytochemical analysis.