Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Identification of a Newly Isolated Streptomycete, Streptomyces sp. SZK1, which Produces Polyene Macrolide Strevertenes

Akinori Ninomiya1 and Shinya Kodani1,2*

1Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan. 2Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.

Received on 02 April 2013 and accepted on 23 May 2013

 

ABSTRACT

Streptomyces sp. SZK1 was isolated from a soil in Japan using ISP2 agar medium. As a result of antimicrobial screening, the acetone extract of the strain SZK1 exhibited promising antimicrobial activities against Saccharomyces cerevisiae, and Schizosaccharomyces pombe. Isolation and identification of the antimicrobial principles from the strain SZK1 was accomplished. As a result of analysis using NMR and MS spectra, the main antimicrobial compound was identified as strevertene A. To identify the strain SZK1, a partial 16S rRNA gene sequence from the strain SZK1 was determined and found to have high identity (99%) with Streptomyces racemochromogenes.

Keywords : Streptomyces, Strevertene, HPLC, NMR Spectra, 16S rRNA gene.