Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Screening and Optimization of Fermentation Media for Increased Production of Ethanol from Gracilaria sp. using Response Surface Methodology

S. Karunakaran1*, R. Gurusamy2, D. Senbagam3 and B. Senthilkumar3

1Department of Biotechnology, Vivekanandha College of Engineering for Women, Tiruchengode - 637 205. Tamil Nadu, India. 2Department of Science and Humanities, Hindustan Institute of Technology, Coimbatore, Tamil Nadu, India. 3Department of Biotechnology, Muthayammal College of Arts and Science, Rasipuram -637 408, Tamil Nadu, India.

Received on 25 September 2013 and accepted on 23 November 2013



The study is aimed at identifying and optimizing physical parameters and composition of fermentation media for enhanced ethanol production from Gracilaria sp. (red macroalgae) by Saccharomyces cerevisiae using response surface methodology. The sea weed was first undergone for acid hydrolysis and enzyme saccharification for releasing of reducing sugar. These pretreated algal hydrolysates were used for optimization study. The variables such as algal hydrolysate, incubation period, Na2HPO4 and pH were found to be significant variables to influence the ethanol production. The identified variables were optimized by Box Benhen design (BBD). The optimum conditions are: algal hydrolysates - 8.5%, incubation period - 1.7 days, Na2HPO4 - 0.3gL-1 and pH - 6.1. These conditions were validated by scale up study which revealed an increased production of ethanol (23.612 gL-1).

Keywords : Biofuel, Ethanol, Seaweeds, Response surface methodology, Optimization and Saccharification.