Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Optimization of the Biosynthesis of the Macrolide Antibiotic WA52 Produced by the Alkaliphilic Isolate Nocardiopsis dassonvillei WA52

Wael N. Hozzein1,2, Mohamed Ibrahim A. Ali3 and Maged S. Ahmed2

1Bioproducts Research Chair (BRC), Zoology Department, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia. 2Department of Botany, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt. 3Department of Botany, Faculty of Science, Cairo University, Giza, Egypt.

Received on 28 September 2013 and accepted on 10 December 2013



The effects of some physical and nutritional parameters were studied for the optimum production of a macrolide antibiotic, designated WA52-A, that was produced in the fermentation culture of the alkaliphilic strain Nocardiopsis dassonvillei WA52. The effects of incubation period, initial pH and incubation temperature were evaluated. The results revealed that the maximum antibiotic production was achieved after 5 days incubation at 30?C with an initial pH of 9. Subsequently, the effects of the nutritional requirements, carbon source, nitrogen source and mineral salts, were investigated. The results indicated that the maximum antibacterial activity was obtained using a fermentation medium with the following composition (g/100ml): Glucose, 2.5; NaNO3, 0.2; K2HPO4, 0.12; MgSO4, 0.05; KCl, 0.06; and FeSO4, 0.008.

Keywords : Optimization, antibiotic, alkaliphilic, Nocardiopsis dassonvillei WA52.