Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Diversity of Cultivable Halophilic/Halotolerant Eubacteria Isolated from Shache, Xinjiang, China and Characteristics of their Hydrolase

Jiewei Tian1,2, Zuchao Lei2, Yang Liu2, Peng Qiu2, Lei Wang2 and Yongqiang Tian1,3,*

1Key laboratory of Leather Chemistry and Engineering, Ministry of Education and College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu - 610 065, China. 2Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, Sichuan - 610 065, China. 3National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu, Sichuan - 610 065, China.

Received on 07 May 2013 and accepted on 18 August 2013

 

ABSTRACT

For the past few years, bacteria that are grown in high-salt environment have been a focus of extremophiles research. They are distributed widely in different habitats and possess unique physiological properties. In this study, we obtained 97 halophilic or halotolerant eubacteria from saline-alkali soil in Shache County, Xinjiang Province, China, by selective media. Phylogenetic analysis of the partial sequences indicated that the isolates were affiliated with 19 genera of three orders. All 97 strains were screened for amylase, lipase, gelatinase and cellulase. Twenty-five strains were lipase producers, 27 gelatinase producers, 31 amylase producers, and none were cellulase producers. Combined hydrolytic activities were detected in some strains. Twenty-one isolates presented with two or more activities, but only five isolates presented with three hydrolytic activities. For the tolerability tests of amylase and lipase, we found that most of the isolates presented the highest amylase activity at 10% NaCl, pH 7.0 and 40?C, and the highest lipase activity at 5-10% NaCl, pH 8.0 and 30?C.

Keywords : Halophiles, Halotolerant Bacteria, Amylase, Lipase, Gelatinase.