Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Molecular Characterization and Antifungal Activity of Pyocyanin (Phz A) Gene

Thukkaram Sudhakar1*, Sundaramurthy Karpagam2 and Usha Sekar3

1Department of Biomedical Engineering, Sathyabama University, Chennai - 600 119 ,India. 2Department of Plant Biology and Biotechnology, Queen Mary's College, Chennai - 600 004, India. 3Department of Microbiology, Melmaruvathur Adi Parasakthi Institute of Medical Science and Research, Melmaruvathur, Kanchipuram - 603 319, India.

Received on 25 July 2013 and accepted on 09 September 2013

 

ABSTRACT

P. aeruginosa, an opportunistic pathogen produces antimicrobial substance in the form of a diffusible pigment, pyocyanin. The pyocyanin pigment was extracted using solvent extraction and further characterization studies were performed. The genotypic confirmation of biosynthetic pyocyanin phz A gene was amplified by PCR using suitable primers. The amplified gene corresponded to 217bp sequence. Further, the antifungal activity of the pigment pyocyanin was assessed using various fungal (yeasts) cultures. The antifungal activity of the pigment was found to contain an inhibitory substance, pyrrolnitrin that helps to arrest the electron transport chain of the tested cultures. This pigment can be tailored for an enhanced antimicrobial efficiency targeting only the pathogenic micro organisms.

Keywords : Pseudomonas, Pyocyanin, phz A.