Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Gibberellic Acid Production by Bacillus cereus Isolated from the Rhizosphere of Sugarcane

N.D. Pandya1 and P.V. Desai2

1Department of Microbiology, Arts, Science and Commerce College, Kamrej Cross Road, Surat - 394 185, India. 2Department of Biosciences, Veer Narmad South Gujarat University, Surat - 395 007, India.

Received on 07 February 2013 and accepted on 02 April 2013

 

ABSTRACT

Gibberellic acid (GA3) producing efficient strain was isolated from rhizospheric soil of sugarcane in the vicinity of Surat city, India and characterized as Bacillus cereus according to standard methods. This isolate was tested for the gibberellic acid production in a Nutrient medium. Optimization of various physiological conditions (inoculum size, incubation temperature, pH of the growth media, incubation period, and incubation condition) were carried out to achieve maximum production of gibberellic acid. The highest 394.18?g/ml of gibberellic acid production was obtained in Nutrient broth after 9 days of incubation at 6.0 pH and 30?C temperature on a rotary shaker with the107 cells/ml of inoculum size.

Keywords : Gibberellic acid, Sugarcane, Bacillus cereus.