Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Molecular Characterization of Lactobacillus plantarum Isolates of Vegetables and Phyllosphere

Y.L. Savitha1*, S.R. Mulla1, D.M. Preethi2, C. Sandeep1, V.C. Suvarna3 and C.K. Suresh1

1Department of Plant Biotechnology, 2Department of Plant Pathology, 3Department of Agricultural Microbiology, University of Agricultural Sciences, GKVK, Bangalore, India.

Received on 08 February 2013 and accepted on 26 March 2013

 

ABSTRACT

The present study was conducted to know the biological and molecular variability among the Lactobacillus plantarum isolates. Different vegetables and phyllospheres were screened for Lactobacillus spp. Out of the fourteen isolates; ten were identified as Lactobacillus plantarum based on microscopic observation, biochemical tests and by using species specific primer. RAPD marker analysis was used to study the molecular diversity. Total of 37 bands were scored in RAPD analysis and 29 bands were found to be polymorphic. RAPD analysis depicted that ten isolates formed four clusters. Banding pattern of these isolates distinguished the isolates of different sources.

Keywords : Lactobacillus plantarum, Molecular diversity and RAPD.