Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Production, Purification and Characterization of Siderophore from Pseudomonas fluorescence

V. Jeyanthi1 and P. Ganesh2

1Department of Zoology, Manonmaniam Sundaranar University, Tirunelveli, India. 2Department of Zoology, Annamalai University, Chidambaram, India.

Received on 18 July 2013 and accepted on 07 September 2013

 

ABSTRACT

In the present study plant growth promoting rhizobacterial strains belonging to fluorescent pseudomonads were isolated from rhizosphere soil and screened for siderophore production. Among these isolates, the potent strain was identified by biochemical and 16S ribosomal DNA gene sequencing and the isolate was confirmed as Pseudomonas fluorescence. Optimized production of siderophore was carried out utilizing various sources such as carbon, nitrogen, organic acids, amino acids and pH (5-9). Siderophore production was optimum during 24-30 h incubatory span at pH 7, 29?C temperature. Other parameters investigated were sugars, nitrogen sources, organic acids, amino acids, metal ions and iron (FeCl3). The siderophore was purified using Amberlite XAD-4 column chromatographic technique. The purified siderophore was characterized by FTIR spectral analysis.

Keywords : Siderophore, Optimized production, Pseudomonas fluorescence, Succinate medium.