Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Utilization of Paddy Husk for Tannase Production under Solid State Fermentation

R. Paranthaman1* and K. Singaravadivel2

1SASTRA University and Indian Institute of Crop Processing Technology, Ministry of Food Processing Industries, Thanjavur - 613 005, India. 2Department of Food Quality and Testing Indian Institute of Crop, Processing Technology, Ministry of Food Processing Industries, Thanjavur - 613 005, India.

Received on 24 July 2013 and accepted on 10 October 2013

 

ABSTRACT

The present work has been taken up with a view of exploring the possibilities of using Agriculture by products with Tannic acid as a source for the production of tannase and optimizing condition require to get maximum production The fungus Aspergillus oryzae produced tannase from the cheap source of Paddy Husk on solid state fermentation. The crude tannase activity was found to be 9.5 U/g/min after Purification of Tannase by ammonium sulphate Precipitation 14.9 U/g/min, Dialysis 23.5 U/g/min and Column chromatography 28.3 U/g/min which increase the enzyme activity.

Keywords : Tannase, Tannic acid, Fermentation, Purification, Paddy Husk powder.