Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Biodegradation of b-lactam Antibiotic 'Ampicillin' by White Rot Fungi from Aqueous Solutions

Ramasamy Rajesh Kumar+, Bong Ju Park1+, Hae Ran Jeong, Jae Taek Lee and Jae Young Cho*

Department of Bioenvironmental Chemistry, Chonbuk National University, Jeonju, Republic of Korea. 1Department of Horticultural Science, Chungbuk National University, Cheongju, Chungcheongbuk-do, 361 763, Republic of Korea.

Received on 08 February 2013 and accepted on 25 March 2013



The worldwide higher usage and release of Pharmaceutical and Personal Care Products (PPCPs) into is an emerging environmental issue as it has adverse effects to humans, animals, plants and microorganisms. Hence it is necessary to find a novel mechanism to degrade and remove these PPCPs from the polluted site. One of the possible methods is employing white rot fungi, as it has the capability of degrading lignin. In this study, use of white rot fungi Verticillium leptobactrum KCTC 26026 to degrade a b-lactam antibiotic ampicillin was investigated. The antibiotic was added at a concentration of 0.5 mg L-1, 1.0 mg L-1 and 2.0 mg L-1 and incubated for 7 days in a laboratory simulated environment. The HPLC-DAD studies have shown 100% depletion of ampicillin. To the best of our knowledge, this is the first study in which white rot fungi Verticillium leptobactrum KCTC 26026 was employed for degrading antibiotic.

Keywords : Biodegradation, Ampicillin, Verticillium leptobactrum, White rot fungi, HPLC.