Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Biological Control of Fruit Lesions Caused by Xanthomonas campestris Pathovars from Cuscuta pedicellata Ledeb. In vitro

Amna Ali*, Muhammad Saleem Haider and Muhammad Ashfaq

Institute of Agricultural Sciences. University of the Punjab, Quaid-E-Azam Campus, Lahore- 54590, Pakistan.

Received on 08 August 2013 and accepted on 17 September 2013

 

ABSTRACT

In search for alternative ways of bacterial fruit lesions control, we evaluated the aqueous decoction and aqueous infusion extract of Cuscuta pedicellata L. for their antibacterial activity against different pathovars of Xanthomonas campestris the causal agent of this disease. The collected leaves were washed, dried and powdered. Aqueous decoction and aqueous infusion extracts were prepared and observed their antibacterial efficacy by using the well diffusion method in vitro. The significant results were obtained show that the both extracts tested inhibited the bacterial growth of pathogen with inhibition zone diameter ranging from 1.0 to 5.0cm. Results evidenced the variation in susceptibility among test pathovars of X. campestris towards the C. pedicellata extracts. The aqueous decoction of C. pedicellata revealed strongest antibacterial activity against X. campestris pv. punicae and X. campestris pv. mangiferaeindicae. On the other hand, aqueous infusion extract exhibited effective antibacterial activity against X. campestris pv. citri and X. campestris pv. pinicae. The present investigation strongly indicates biological potential of C. pedicellata against different pathovars of Xanthomonads in the sustainable bacterial disease management in agriculture.

Keywords : Antibacterial activity, Cuscuta pedicellata, Xanthomonads.