Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Facilitation of Paenibacillus amyloliquefaciens on Morchella angusticeps

Chao-Nan Li1, Lin-Hui Li1*, Hai-Jun Liao1, Ting He1, Yan Sheng1 and Bin-Han Li2

1College of Life Science, China West Normal University, NanChong, Sichuan - 637 002, China. 2TongHui Institute of Bio-Science and Technology and Information, NanChong City, Sichuan - 637 002, China.

Received on 08 March 2013 and accepted on 21 April 2013

 

ABSTRACT

The study about the effect on Morchella angusticeps (M. angusticeps) caused by Paenibacillus amyloliquefaciens, P. amyloliquefaciens isolated from the stem base soil of M. angusticeps was performed and significant facilitation on mycelia growth and exoenzyme secretion of M. angusticeps happened. The result of experiments showed that the growth rate of M.angusticeps mycelia peaked on 5th day, reaching 21.92%, when mycelia were treated with 20% fermentation broth of P. amyloliquefaciens (FBPa). Further studies conclusively pointed out that the facilitation was involved with extracellular enzyme of M. angusticeps according to its soluble protein concentration, keeping high-level during the 5 days. High-level activities were detected in laccase and protease determination when FBPa concentration increased to the range of 10% to 20%, and in amylase and cellulase determination when it reached 20% on 5th day.

Keywords : Morchella angusticeps, Paenibacillus amyloliquefaciens, Rhizobacteria, facilitation, exoenzyme.