Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Anthraquinone Metabolites from Endophytic Nigrospora sp BM-2 and Optimization of Fermentation Medium for High Bostrycin Production

Hongqi Zhang1, Zhangshuang Deng2*, Zhiyong Guo2, Jianfeng Chen2, Junzhi Wang2 and Kun Zou1,2*

1School of Basic Medical Sciences, Wuhan University, Wuhan - 430 072, China. 2Hubei Key Laboratory of Natural Products Research and Development, College of Chemistry and Life Science, China Three Gorges University, Yichang - 443 002, China.

Received on 03 July 2013 and accepted on 22 August 2013

 

ABSTRACT

Bioassay-guided separation led to identification of bostrycin1, which was a potentially promising anti-tumor drug candidate, together with other four anthraquinone 2-5 derivatives from liquid culture of endophytic Nigrospora sp. BM-2 isolated from Saccharum arundinaceum Retz. In order to obtain high bostrycin production, three single-factor experiments and a L9(34) orthogonal matrix experimental design have been employed in the cultivation of Nigrospora sp. BM-2. The optimal fermentation medium were glucose 20.0 g/L, yeast extract 8.0 g/L, MgSO4 0.05 g/L, KH2PO4 0.01g/L, NaCl 0.5 g/L. After 9 days of incubation under shake flask conditions at 120 rpm and 28 ?C in optimal media, the highest amount of bostrycin was 86.25 ? 7.23 mg/L in culture broth. Furthermore, enzymatic inhibitor as regulate factor in the biosynthesis of bostrycin was attempted to give adverse effects.

Keywords : Endophytic Nigrospora sp., Anthraquinone, Bostrycin, Optimization, Medium.