Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Production of Biological Control Agent Bacillus subtilis B579 by Solid-State Fermentation using Agricultural Residues

Tao Tian1, Bing Bing Sun1, Shu Qin Sun1, Xiu-Rong Yang1, Wei Li1, Ping Ma2* and Li-Qun Zhang3

1Institute of Plant Protection, Tianjin Academy of Agricultural Sciences, Tianjin 300381, P. R. China. 2 Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding, Hebei 071000, P. R. China. 3 Department of Plant Pathology, China Agricultural University, Beijing, 100193, P. R. China.

Received on 30 March 2013 and accepted on 21 May 2013

 

ABSTRACT

Three different agricultural residues were evaluated as basic substrates for the production of biological control agent (BCA) Bacillus subtilis strain B579, by solid-state fermentation (SSF). Among them, wheat bran (WB) was shown to be the most suitable substrate, and an initial moisture content of 57% was determined to be optimal for SSF of strain B579. Moreover, employing the Plackett-Burman and Box-Benhnken experimental design models, nine different supplements were investigated to maximize the cell yield. Results demonstrated that a relatively maximal cell yield of 2.12?0.03?1011 CFU/g was achievable utilizing WB as the basic medium supplemented with beef extract (21.3 g kg-1), cornmeal (42.6 g kg-1), and soluble starch (23.8 g kg-1).

Keywords : Agricultural residues, Bacillus subtilis B579, Biological Control agent, Design-Expert, Solid-State Fermentation.