Journal of Pure and Applied MicrobiologyVol. 2 No. 2

Study on the Amylolytic Activity of Bacteria Isolated from Spent Mushroom Substrate

R. Kumuthakalavalli, S. Surumbar Kuzhali and S. Reka

Department of Bilogy, Gandhigram Rural University, Gandhigram - 624 302, India.

Received on 25 March 2008 and accepted on 12 June 2008



Seven different bacterial strains were isolated from spent mushroom substrate (SMS) and tested for starch hydrolysis. Out of them, three bacterial strains namely Bacillus spp (B1), Pseudomonas spp, Bacillus spp (B2) were able to form clear zone in starch hydrolyzing medium. These three strains were subjected for amylase assay and extra cellular amylase production was observed as 214,180 and 245µg/ml respectively.

Keywords : SMS, Starch hydrolysis, Bacillus, Pseudomonas, extra cellular amylase.