Journal of Pure and Applied MicrobiologyVol. 2 No. 2

Development and Validation of a Spectrophotometric Method for Estimation of Thyroxine Sodium in Bulk and in Tablet Dosage form

C. Bala Sekaran1*, A. Prameela Rani2, B.B. Pawan Kumar3, P.S.K. Bhargav3 and B.N.V.R. Kinnera3

¹Department of Biotechnology, ³Department of Microbiogy, P. B. Siddhartha College of Arts and Science, Vijayawada - 520 010, India. ²Department of Pharmaceutics, K.V.S.R. Siddhartha College of Pharmaceutical Sciences, Vijayawada - 520 010, India.

Received on 06 April 2008 and accepted on 15 August 2008



A simple and sensitive spectrophotometric method for the determination of thyroxine sodium was developed. This method was based on condensation of the primary amino group of thyroxine sodium with acetyl acetone and formaldehyde producing a yellow coloured product, which is measured spectrophotometrically at 430nm. The colour was stable for at least 5 hours. Beer’s law was valid within a concentration range of 10 - 50 µg/mL. No interference was observed in the presence of common pharmaceutical excipients. The validity of the method was tested by analyzing thyroxine sodium in its pharmaceutical preparations. Good recoveries were obtained. This method was successfully employed for the determination of thyroxine sodium in various pharmaceutical preparations and biological samples.

Keywords : Tyroxine sodium; spectrophotometric analysis; acetyl acetone/ formaldehyde reagent