Journal of Pure and Applied MicrobiologyVol. 2 No. 2

Isolation and Characterization of Bacillus thuringiensis Strains from the Ecosystem of Central India

Sarita Agrahari1, M.P. Moharil2*, Archana pethe1 and N.G.V. Rao²

¹Department of Microbiology, Shri Shivaji College, Akola, India. ²Insect Biotechnology Laboratory, Biotechnology Centre, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, India.

Received on 06 April 2008 and accepted on 15 August 2008



The importance of Bacillus thuringiensis (Bt) in crop pest management is now well established. Acting as an environmentally safe manner, Bt is an alternative to many chemical insecticides and for the control of chemically resistant insect pests. Bt is ubiquitous soil bacteria can be isolated readily from soil, insects, stored product material, sericulture environment, the phylloplane etc. The aim of this study was the isolation of Bt from agroecology of central India. Physiological, morphological and biochemical characters were studied for isolation and identification of different Bacillus thuringiensis strains in terms of their protein toxicity to the crop pests.

Keywords : Bacillus thuringiensis (Bt), Isolation, Ecosystem.