Journal of Pure and Applied MicrobiologyVol. 7 No. 3

Diagnosis of Enteric Fever by Widal and Two Dot-enzyme Immunoassay: Utility and Difficulties

Manjunath A Hosamani1*, Asha B Patil2, Shobha D Nadagir3, N.S. Madhusudhan4 and Pramod Sambrani5

1Department of Microbiology, SDM Medical College & Hospital, Sattur, Dharwad, India. 2,3,5Department of Microbiology, Karnataka Institute of Medical Sciences, Hubli, India. 4Department of Microbiology, Indira Gandhi Medical College & Research Institute, Pondicherry, India.

Received on 18 October 2012 and accepted on 21 December 2012



Enteric fever is an important cause of mortality and morbidity in developing and under developed countries. The present study was undertaken to detect antibodies to salmonella using serological tests i.e. Two dot-enzyme immunoassay (Typhidot) and Widal test and correlation of the results. Consecutive 100 blood samples collected from clinically suspected cases of enteric fever were processed using standard methods. The samples were subjected to blood culture, widal test & typhidot test. Blood culture was positive in 7(7%), Widal test positive in 20(20%) and Typhidot in 28(28%). The sensitivity, specificity, PPV and NPV for Typhidot and Widal when compared with blood culture as gold standard were 100%, 77.41%, 25%, 100% and 85.71%, 84.95%, 30%,98.75% respectively. Typhidot test being rapid, inexpensive, easy to perform and highly sensitive test, has an advantage over conventional methods like blood culture and widal test in diagnosis of enteric fever caused by salmonella typhi. Typhidot is also more useful in detection of enteric fever in the 1st and 2nd week of infection.

Keywords : Enteric fever, Widal test, Typhidot test.