Journal of Pure and Applied MicrobiologyVol. 2 No. 2

Production, Purification and Application of Collagenase

Abirami N. and Priya R. Iyer*

Women‘s Christian College, College Road, Chennai - 600 006, India.

Received on 18 May 2008 and accepted on 23 July 2008

 

ABSTRACT

A bacterial strain of Staphylococcus aureus producing collagenase was isolated from scales of fish and characterized. Optimum growth requirements of the organism for production and activity of the enzyme were determined. The collagenase was purified by ammonium sulphate precipitation method and dialysis. The molecular weight of the partially purified enzyme was estimated to be 62 kDa by NATIVE-PAGE. The enzyme was also immobilized by Sodium alginate gel entrapment method and the enzyme was used in tenderization of meat.

Keywords : Collagenase, Staphylococcus aureus.