Journal of Pure and Applied MicrobiologyVol. 2 No. 2

Efficacy of Chitosan Against Selected Human Pathogens Under Different Environments

S. Murugan¹*, P. Uma Devi² and S. Suja³

¹PG and Research Department of Microbiology, ³PG and Research Department of Biochemistry, Dr. N.G.P. Arts and Science College, Coimbatore - 641 048, India. ²School of Biotechnology, Karunya University, Coimbatore - 641 114, India.

Received on 10 April 2008 and accepted on 18 August 2008



The aim of the study is to evaluate the antimicrobial activity of chitosan in hydrochloric and lactic acids against nine different microorganisms: Methicillin Resistant Staphylococcus aureus (MRSA), Methicillin Sensitive Staphylococcus aureus (MSSA), Salmonella typhi, S. paratyphi A, S. typhimurium, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Proteus vulgaris. Using well diffusion method performed antimicrobial screening. Studies with chitosan- HCl, chitosan- lactic acid, chitosan-lemon and chitosan-garlic extracts indicated that the growth of tested organisms was markedly inhibited. Chitosan lactic acid enhanced the antimicrobial activity than chitosan-HCl, similarly chitosan-lemon and chitosan- garlic extracts exhibited higher inhibitory effect than the chitosan –HCl and lactic acid. Efficacy of chitosan under different environment conditions shows variable inhibitory activity against each bacterium tested. The present study finding confirms the antimicrobial action of chitosan against the drug resistant bacterial strains and typhoid bacteria.

Keywords : Chitosan- HCl, Chitosan- lactic acid, synergistic effect- lemon and garlic extracts