Journal of Pure and Applied MicrobiologyVol. 2 No. 2

Isolation and Characterisation of Enzyme Invertase from Halotolerant Yeast

R. C. Patil¹, U. Jadhav, A. Mule¹, E. Gorwalla¹, A. M. Parekh² and V. M. Jadhav²

1Department of Microbiology, Bhavan’s College, Andheri (W.), Mumbai - 400 058, India. Department of Life Sciences, University of Mumbai, Santacruz(E), Mumbai - 400 098, India. ²Bharati Vidyapeeth’s College of Pharmacy, Navi Mumbai - 400 614, India.

Received on 06 April 2008 and accepted on 18 July 2008

 

ABSTRACT

Sixty-seven pure cultures of halophilic bacteria and yeasts were isolated from Thane creek water, soil, and other materials. High salinities (15% NaCl) were maintained during all phases of the isolation procedure to avoid possible destruction of obligate halophiles. Four of the cultures, all yeasts, proved to be obligate halophiles. Three are species of Candida and one of Torulopsis. They grew in the range of 10% to 20 % salinity and the optimum salinity was 15 %. They died rapidly when exposed to lower saline conditions between 0-5%.

Keywords : Invertase, halophilic bacteria, halotolerant yeast.