Journal of Pure and Applied MicrobiologyVol. 7 No. 2

Antimicrobial Potentiality of Elsholtzia densa against Pathogenic Bacterial and Fungal Strains

Misba Khan1, Showkat Ahmad Ganie1, Ishfak Hussain Wani1, Bashir Ahmad Ganai1, Akhter Hussain Malik2, Mohammad Afzal Zargar1, Akbar Masood1 and Rabia Hamid1*

1Department of Biochemistry, University of Kashmir, Srinagar - 190 006, India. 2Department of Botany, University of Kashmir, Srinagar - 190 006, India.

Received on 03 January 2013 and accepted on 23 February 2013

 

ABSTRACT

The present study was proposed to evaluate the antimicrobial efficacy of Elsholtzia densa, a rare annual herb of Kashmir valley. Five bacterial strains Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Shigella flexneri and Salmonella typhi and three fungal strains Penicillium notatum, Acremonium alternatum and Aspergillus niger were tested for their susceptibility to Elsholtzia densa extracts. All the extracts of Elsholtzia densa with a concentration of 10% (methanol, ethanol, butanol, ethyl acetate and aqueous) were tested for antimicrobial activity by the disc diffusion assay. The total phenolics concentration was found to be 62.5mg% and 77.5mg% in case of absolute ethanolic and 50% ethanolic extracts respectively. However, only ethyl acetate extract showed antimicrobial activity which was visible as the zones of inhibition formed in the different cultures of Gram positive and Gram negative bacteria as well as in case of fungal cultures. The maximum activity was seen for Staphyloccus aureus with inhibition zone diameter of 23.66 2.08 mm followed by Escherichia coli with inhibition zone diameter of 20.33 2.51 mm. The extract had the highest MIC and MBC (4mg/ml) for Staphylococcus aureus and Salmonella typhi while a lowest MIC of 2 mg/ml and MBC of 1.8 mg/ml was shown for Shigella flexneri. In case of antifungal activity, the maximum inhibition zone diameter of 8.00 2.00 mm was observed for Acremonium alternatum followed by 17.33 1.15 mm for Aspergillus niger and 16.33 1.15 mm for Penicillium notatum. The MICs and MBCs for the fungal strains ranged between 2.0 4.0 mg/ml. The purpose of screening was to justify, authenticate and validate the use of this herb as a traditional medicine in the treatment of infectious diseases.

Keywords : Elsholtzia densa extracts, Antimicrobial activity, Bacterial & Fungal strains.