Journal of Pure and Applied MicrobiologyVol. 2 No. 2

Detection of Protease Production by Plate Assay, Submerged Culture and Solid State Fermentation: Statistical Comparison and Significance

Ulhas Patil and Ambalal Chaudhari¹*

Department of Microbiology, R. C. Patel A. S. C. College, Shirpur - 425 405, India. ¹School of Life Sciences, North Maharashtra University, Jalgaon - 425 001, India.

Received on 12 April 2008 and accepted on 28 May 2008



Present investigation describes isolation, screening and primary level characterization of 24 protease producing bacterial strains, obtained from diverged geographical locations like alkaline soil, hot spring, soda lake and dairy, solvent and sugar industry effluent. The protease production efficiencies of isolated strains were detected by skim milk agar plate assay. Each isolated strain was analyzed for protease production by broth assay after growing the bacterial strain by submerged culture method. Majority of strains produces large zone on skim milk agar were not imitate the protease yield in submerged culture (Correlation coefficient – 0.049). The protease production abilities were also evaluated by cultivating each strain by solid state fermentation. We found that zone of clearance due to proteolysis can be correlated with protease production by solid state fermentation (Correlation coefficient – 0.9726).

Keywords : Protease, Submerged culture, Solid sate fermentation, Correlation coefficient.