Journal of Pure and Applied MicrobiologyVol. 7 No. 2

Statistical Optimization of Medium Composition for Alginate Lyase Production by Pseudoalteromonas tetraodonis Strain QZ-4 using Response Surface Methodology

Haiqing Tang1,2, Chongrong Ou3 and Xiaodong Zheng1*

1College of Biosystems Engineering and Food Science, Zhejiang University, Zhejiang Hangzhou - 310 029, China. 2Ningbo Entry-exit Inspection and Quarantine Bureau, Zhejiang Ningbo - 315 012, China. 3School of Marine Sciences, Ningbo University, Zhejiang Ningbo - 315 211, China.

Received on 10 January 2013 and accepted on 02 March 2013

 

ABSTRACT

The effect of medium composition on alginate lyase production, by a lineage of Pseudoalteromonas tetraodonis, was investigated using response surface methodology. The concentrations of inorganic nitrogen and salts in culture medium, such as di-ammonium hydrogen phosphate, sodium chloride, ferrous sulfate, magnesium sulphate and potassium di-hydrogen phosphate, were changed to obtain the optimal production. The result showed that di-ammonium hydrogen phosphate, sodium chloride and magnesium sulphate had significant effect on alginate lyase production using a two-level fractional factorial design (FFD). The maximum yield of alginate lyase was attained in a medium containing (g/L): Alginate sodium 5, (NH4)2HPO4 11.6, NaCl 32.9, FeSO4 0.03, MgSO4 0.08 and K2HPO4 0.4, using the surface response methodology and central composite design (CCD). The model prediction of alginate lyase yield at 145.45 U/mL was experimentally verified.

Keywords : Alginate lyase, Fermentation, Response surface methodology (RSM), Two-level fractional factorial design (FFD), Central composite design (CCD).