Journal of Pure and Applied MicrobiologyVol. 7 No. Special Edition

The Effects on Degradation Treatment of CMC-HLC/EDC-ADH Hydrogels using Collagenase I and Simulated Body Fluid

Xian Li1*, Daidi Fan1*, Jianjun Deng1, Junfeng Hui1, Xiaoxuan Ma1, Chenhui Zhu1 and Wenjiao Xue2

1Shaanxi Key Laboratory of Degradable Biomedical Materials, Department of Chemical Engineering, Northwest University, Taibai North Road 229, Xi’an, Shaanxi 710069, China 2Shaanxi Provincial Institute of Microbiology, No. 76 Xiying Road, Xi’an 710043

Received on 03 March 2013 and accepted on 14 April 2013



A novel hydrogel for biomedical applications was successfully fabricated by human-like collagen (HLC) and carboxymethylcellulose (CMC) with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and adipic acid dihydrazide (ADH) as cross-linkers. The interior morphologies of these hydrogels were also characterized before and after degradation by collagenase I and simulated body fluid (SBF). The results of in vitro degradability tests of these hydrogels that the CMC-HLC/EDC-ADH hydrogel in SBF indicated that possesses slow degradability than in collagenase I in vitro. Therefore, the results provided the possibility that CMC-HLC/EDC-ADH hydrogels are suitable for biomedical applications such as soft tissue augmentation for their good biocompatibility.

Keywords : Hydrogel, Collagens I and Simulated body fluid