Journal of Pure and Applied MicrobiologyVol. 2 No. 2

Detection of Extended Spectrum Beta Lactamases (ESBLs) in Surgical Wound and Burn Infections

A. Umasankar¹*, T. Shobarani² and D. Satyaranjan¹

¹Department of Microbiology, College of Science, GITAM Deemed University. Vishakapatnam - 530 045, India. ²Department of Microbiology, Rangaraya Medical College, Kakinada, India.

Received on 12 June 2008 and accepted on 30 July 2008

 

ABSTRACT

Extended spectrum beta lactometers are the enzymes, which were mainly occurred in hospital acquired infections (nosocomial infections) due to unhygienic conditions of the hospital environment. ESBL production is usually plasmid mediated, it is possible for one specimen to contain both ESBL producing and non ESBL producing cells of the same species. This suggests that for optimal detection, several colonies must be tested from a primary culture plate. In the process of detection of these enzymes, first we isolate the organism from wound based on the culture characters and biochemical characters and proceed for antibiotic sensitivity. If the organism shows resistant, then we have proceed for test for Beta lactamases, and confirmatory tests for ESBLs.The prevalence of ESBLs in government general hospitalk kakinada and surroundings places in kakinada district is 9.33%. It is much lower than other reports from India and abrod. In our study surprisingly out of 21% of esbl positive cases, 16% of the isolates were Proteus species. All the isolates were found sensitive to the Carbapenem antibiotics.

Keywords : ESBLs - Extended Spectrum Beta-Lactamases, 3GC-third generation cephalosporins